ABSTRACT
In the present study a series of experiments was performed under battery condition, two groups of chicks of 4 weeks age were vaccinated with mixed oocyst at high and low level once was kept free of infection as a control following a challenge infection to each group the result showed an established immunity compared to their respective uninfected control. In recent years report of coccidia vaccine developing showed that the best way of vaccination is administered to chicks in the water. In order to develop the vaccine diluent, the mixture of some gelatin used to correct the density of solution and on this basis the oocyst mixture is properly mixed in the diluent. The results demonstrated that as long as chick have enough appetite dietery supplementation with vitamin E 100mg/kg feed can prevent oocyst shedding compared to the control group which did not receive vitamin E. It seems the development of immunity of vaccinated chicks was increased when vitamin E added at level above those recommended as nutritional requirements